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Initially PCR was executed by using exon 4a-specific ahead primer (4a-F, 5'ATTCCATTGCCTTTCCACAG-3') and 3' RACE outer reverse primer (5'-GCGAGCACAGA ATTAATACGACT-3'). Nested PCR was performed through the use of the primary PCR product or service like a template together with the nested Ex4a(+)WT1 cDNA-specific ahead primer (4a-F2, 5'-GCAAAGCTCT CTGTG ACATTA-3') as well as nested adaptor interior p