Bated at 1:10,000 dilutions for 1 hr and blots developed using Clarity Western ECL Substrate kit (Bio-Rad).Flow cytometryFor cell surface marker staining, Jurkat cells were washed twice with PBS/0.5 BSA, and incubated with 50 ml of the antibody diluted in PBS/0.5 BSA for 30 min in the dark. Antibodies used include CD69-PE (12?699, eBioscience, San Diego, CA) or Mouse IgG1 K isotype control PE (1