Of eight manage genes (B2M, B4gAlT1, ClTC, e2F4, gAPDH, POlr2A, SDHA, and TBP) by taking the ratios of every gene's counts per sample to the typical across all samples and scaling by the median of these ratios in each sample. This normalization issue was also applied to the unfavorable control probes counts. A detection threshold was defined for each sample as 5 instances the mean on the adverse c